Peptide abundance correlations in metaproteomics enhance taxonomic and functional analysis of the human gut microbiome

In this study, we analyzed peptide abundance correlations within a metaproteomics dataset derived from in vitro cultured human gut microbiomes subjected to various drug treatments.

Our analysis revealed that peptides from the same protein or taxon exhibited correlated abundance changes.

In species representative genome subsets, peptide correlation networks based on taxon-normalized peptide abundance (TNPA) linked functionally related peptides and provided insights into uncharacterized proteins.

Altogether, our study demonstrates that analyzing peptide abundance correlations enhances both taxonomic and functional analyses in human gut metaproteomics research.